OXP-17 Absorption and Emission

Topics

Lambert-Beer Law
Absorbance
Fluorescence Dichroitic Filter
Phosphorescence
Absorption / Emission Spectra


Examples of Investigation and Measurement

Lambert-Beer Law
In a first measurement the influence of absorbing material on the intensity of transmitted light is investigated. Parameters like thickness of the sample or the wavelength of the light source are varied and the transmission is detected by a photodiode.

Absorption spectrum
With a white light source and an optical grating the spectral distribution of the absorption profile is visualized and can be observed.

Fluorescence spectrum
Slight changes in the set-up allow the excitation of fluorescing samples with LEDs of different wavelengths. The spectra of absorption and fluorescence are investigated by means of an optical grating.

Phosphorescence
A phosphorescing sample is tested for its wavelength sensitivity. With a photo detector the phosphorescence decay is measured as a function of time.
 
 


Required Equipment

Cat. No.     Qty.     Description
02.0500      1          Profile rail, 500 mm
02.1022      1          Screen holder, carrier 25
02.1024      2          Plate holder, carrier 25
02.1602      1          Optical screen, 80x65, scale
02.2126      1          Mounting plate, click 25, carrier 20
02.2132      3          Mounting plate, click 30, carrier 20
02.6106      1          Adjustment holder, 4 axes, carrier 20
04.0060      1          Plano convex lens f=40 mm, 25.4, click 30
04.0062      2          Plano convex lens f=60 mm, 25.4, click 30
04.0104      1          Phosphorescing sample, click 25
04.0194      1          Set of 3 fluorescent filters
04.0702      1          Optical grating, 600 lines / mm
05.0032      1          High brightness LED lamp, red
05.0036      1          High brightness LED lamp, green
05.0038      1          High brightness LED lamp, blue
07.0010      1          BNC - banana adapter connection leads 2x4 mm plugs
07.0102      1          Photodetector, Si PIN, in housing
07.0206      1          Active power supply LDD-05
09.2720      1          Absorption unit
19.0400      1          Digital multimeter 3 ½ digits